Rat Kallikrein 3 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is intended for the in vitro quantitative determination of rat Kallikrein 3/KLK3 in serum, plasma, tissue homogenates or other biological fluids. The procedure uses a pre-coated microplate (12 x 8 well strips) bearing an antibody specific for Kallikrein 3/KLK3, followed by incubation with a biotinylated anti-Kallikrein 3/KLK3 antibody, HRP-avidin conjugate, and TMB substrate, with absorbance read at 450 nm.

The stated assay range is 0.156-10 ng/ml with a sensitivity of <0.057 ng/ml, and the total assay time is 4h 30m. Included components are a pre-coated 96-well microplate, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers. Storage conditions range from +4°C to -20°C depending on the component, and the kit must not be used past its expiration date.

Sample data show for serum (n=5) an average of 91% across a range of 80%-102%, for EDTA plasma (n=5) an average of 90% across 81%-100%, and for heparin plasma (n=5) an average of 84% across 80%-89%. Dilution linearity percentages across 1:2, 1:4, 1:8, and 1:16 dilutions are also listed for each sample type.