Rat Interleukin 17F ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com for the in vitro quantitative determination of rat IL-17F in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, or other biological fluids. The kit uses a pre-coated 96-well microplate (12 x 8 well strips), a Biotinylated Anti-IL-17F detection antibody, HRP-Avidin conjugate and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader. The stated product range is 15.6–1000 pg/ml, sensitivity is <5.7 pg/ml, and the total assay time is 4 h 30 min.

Supplied components include a pre-coated microplate, lyophilized standard, Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and adhesive plate sealers. Storage is at +4 °C or –20 °C as instructed, and the kit should not be used past its expiration date. Reported average recovery values are 91% for serum, 95% for EDTA plasma and 96% for heparin plasma, with dilution linearity validated from 1:2 to 1:16 across the three sample matrices.