Rat Insulin Like Protein 3 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com, designed for the in vitro quantitative determination of rat INSL3 in serum, plasma or other biological fluids. The kit uses a pre-coated microplate arranged as 12 x 8 well strips and employs a biotinylated anti-INSL3 antibody followed by HRP-avidin detection with TMB substrate; absorbance is read at 450 nm in a microplate reader.

Reported sensitivity is <0.062 ng/ml and the assay range is 0.234–15 ng/ml, with a total assay time of 4 hours 30 minutes. Average recovery values are 91% for serum (80–102% range, n=5), 90% for EDTA plasma (81–100%, n=5) and 84% for heparin plasma (80–89%, n=5). Supplied components include a pre-coated 96-well microplate, lyophilized standard (2 vials), Detection Solution A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers, with storage at +4°C or -20°C as instructed.

For research use only; not intended for diagnostic or therapeutic use.