Rat Insulin Like Growth Factor 1 ELISA Kit

From Antibodies.com, a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) developed for the in vitro quantitative determination of rat Insulin Like Growth Factor 1 (IGF1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Key performance characteristics include a sensitivity of <30 pg/ml and a working range of 78.1–5000 pg/ml. Detection is colourimetric, with absorbance measured at 450 nm using a microplate reader. The total assay time is 4 hours 30 minutes. The platform is a pre-coated microplate configured as 12 x 8 well strips, and kit components are stored at +4°C or –20°C as instructed.

Reported values (n=5) are 80%–102% with an average of 91% for serum, 81%–100% with an average of 90% for EDTA plasma, and 80%–89% with an average of 84% for heparin plasma.