Rat Indoleamine-2,3-Dioxygenase ELISA Kit

Antibodies.com supplies a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) for the in vitro quantitative determination of rat indoleamine 2,3-dioxygenase. The kit is intended for use with serum, plasma, tissue homogenates, or other biological fluids, and uses a pre-coated 96-well microplate arranged as 12 x 8 well strips.

Standards and samples are incubated with the immobilised capture antibody, then with a biotinylated anti-indoleamine 2,3-dioxygenase detection antibody, followed by an HRP conjugate and TMB substrate for colorimetric detection. The enzymatic reaction produces a blue product that turns yellow after addition of acidic stop solution, and the absorbance is read at 450 nm in a microplate reader, with analyte concentration calculated against the standard curve. The reported product range is 0.156–10 ng/ml, with a sensitivity of <0.053 ng/ml and a total assay time of 4 h 30 m. Recovery ranges reported in the supplied data are 80–102% for serum (n=5), 81–100% for EDTA plasma (n=5), and 80–89% for heparin plasma (n=5).

Kit components include lyophilised standard, detection solutions, wash buffer, sample dilution buffer, TMB substrate, stop solution, plate sealers, and the pre-coated microplate, stored at +4 °C or –20 °C as instructed. Components should not be used past the stated expiration date.