Rat IgG ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay is designed for the in vitro quantitative determination of rat IgG. The assay uses a quantitative sandwich ELISA format in which a monoclonal antibody specific for rat IgG is pre-coated onto a microplate; standards and samples are added, unbound material is washed away, and an HRP-linked detection antibody specific for IgG is applied before substrate addition and color development proportional to bound IgG.

The kit is intended for use with cell culture supernatant, serum and plasma, and employs colorimetric detection on a microplate platform. According to the manufacturer specification, the assay range is 1.56 - 100 ng/ml with a sensitivity of 0.20 ng/ml. The kit should be stored at +4°C and not used past the expiration date, and the manufacturer describes it as a ready-to-use ELISA kit with an easy-to-follow protocol.