Rat Histidine Rich Glycoprotein ELISA Kit

Manufactured by Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit is designed for the in vitro quantitative determination of rat Histidine Rich Glycoprotein (HRG) in serum, plasma, or other biological fluids. The assay uses a pre-coated 96-well microplate configured as 12 x 8 well strips, with detection based on a Biotinylated Anti-HRG Antibody, an HRP-Avidin conjugate, and TMB substrate, read colorimetrically at 450 nm in a microplate reader.

Reported performance figures include a sensitivity of <0.069 ng/ml and an assay range of 0.156-10 ng/ml, with a total assay time of 4h 30m. Sample recovery values are listed as serum (n=5) at 80% - 102% with an average of 91%, EDTA plasma (n=5) at 81% - 100% with an average of 90%, and heparin plasma (n=5) at 80% - 89% with an average of 84%. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.