Rat Growth Regulated Oncogene Beta ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of rat Growth Regulated Oncogene Beta (CXCL2) in serum, plasma or other biological fluids. The assay uses a pre-coated 96-well microplate configured as 12 x 8 well strips, with a biotinylated anti-CXCL2 detection antibody, HRP-avidin conjugate, and TMB substrate; absorbance is read at 450 nm on a colorimetric microplate reader. The stated assay range is 15.6–1000 pg/ml and sensitivity is <5.7 pg/ml, with a total assay time of 4h 30m. Reported recovery in serum (n=5) averages 91% (range 80%–102%), in EDTA plasma 90% (81%–100%), and in heparin plasma 84% (80%–89%), with linearity data provided across 1:2 to 1:16 dilutions for each sample type. Kit components are stored at +4°C or –20°C as indicated, and the kit should not be used past its expiration date.