Rat Glutamate Cysteine Ligase, Modifier Subunit ELISA Kit

Manufactured for Antibodies.com, this Sandwich ELISA kit is intended for the in vitro quantitative determination of rat Glutamate Cysteine Ligase, Modifier Subunit (GCLM) in tissue homogenates or other biological fluids [S1]. The assay employs a pre-coated 96-well microplate arranged as 12 x 8 well strips, and the colorimetric reaction is read on a microplate reader at 450 nm [S1].

Reported performance characteristics include an assay range of 0.156-10 ng/ml and a sensitivity of <0.052 ng/ml [S1]. The total assay time is approximately 4 hours 30 minutes [S1]. In spike-and-recovery testing across five replicates, average recoveries were 91% in serum (range 80-102%), 90% in EDTA plasma (range 81-100%), and 84% in heparin plasma (range 80-89%) [S1]. Linearity was demonstrated across serial dilutions (1:2 to 1:16) for serum, EDTA plasma, and heparin plasma [S1].

Supplied components include a pre-coated microplate, lyophilized standard, Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers, to be stored at +4°C or -20°C as instructed on the component labels [S1].