Rat Forkhead Box Protein P3 ELISA Kit

Manufactured for Antibodies.com, this Rat FOXP3 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of rat FOXP3 in tissue homogenates, cell lysates, or other biological fluids. The assay uses a pre-coated microplate (12 x 8 well strips) and is read on a colorimetric microplate reader at 450 nm, with the concentration of FOXP3 calculated against a standard curve.

The assay has a stated range of 0.312–20 ng/ml, a sensitivity of <0.115 ng/ml, and a total assay time of 4 hours 30 minutes. Recovery has been evaluated across serum (n=5, average 91%, range 80–102%), EDTA plasma (n=5, average 90%, range 81–100%), and heparin plasma (n=5, average 84%, range 80–89%), with sample dilution linearity reported at 1:2, 1:4, 1:8, and 1:16.

The kit includes a pre-coated 96 well microplate, lyophilized standard, Detection Solution A, Detection Solution B, Wash Buffer (30X), Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers. Kit components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.