Rat Fibroblast Growth Factor 2, Basic ELISA Kit

A sandwich enzyme-linked immunosorbent assay (ELISA) from Antibodies.com is intended for the in vitro quantitative determination of rat Fibroblast Growth Factor 2 (basic) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids [S1]. The 96-well microplate is pre-coated with an antibody specific for FGF2, and bound analyte is detected using a biotinylated anti-FGF2 antibody, an HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader [S1].

Reactivity is specific to rat samples [S1]. The quantitative range covered by the standard curve is 15.6–1000 pg/ml, with a reported sensitivity of <4.37 pg/ml [S1]. Total assay time is 4h 30m [S1]. Reported spike-and-recovery values are 80–102% (average 91%) for serum (n=5), 81–100% (average 90%) for EDTA plasma (n=5) and 80–89% (average 84%) for heparin plasma (n=5) [S1]. The product is classified under the Assays and Kits category at Antibodies.com [C0]. Kit components supplied include a pre-coated 96-well microplate (12 x 8-well strips), lyophilised standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, with storage at +4°C or -20°C as instructed [S1].