Rat Fetuin B ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA is designed for the in vitro quantitative determination of rat Fetuin B in serum, plasma, or other biological fluids. The assay uses a pre-coated microplate and employs the sandwich enzyme immunoassay technique, with a biotinylated anti-Fetuin B detection antibody, HRP-avidin conjugate, and TMB substrate read at 450 nm.

Reactivity is limited to rat samples. The reported assay range is 31.2-2000 ng/ml with a sensitivity of <9.4 ng/ml, and the total assay time is 4 h 30 min. Detection is colorimetric and intended for use with a microplate reader. Reported recovery ranges include serum 80-102 % (average 91 %), EDTA plasma 81-100 % (average 90 %), and heparin plasma 80-89 % (average 84 %), each with n=5. Kit components are stored at +4 °C or -20 °C as indicated on the labels, and the kit should not be used past its expiration date.