Rat Epiregulin ELISA Kit

Manufactured by Antibodies.com, this Rat Epiregulin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) intended for the in vitro quantitative determination of rat Epiregulin in serum, plasma, tissue homogenates, cell culture supernates, or other biological fluids. The assay is read using a colorimetric microplate reader.

The product working range is 15.6-1000 pg/ml with a sensitivity of <6.1 pg/ml, and the total assay time is 4h 30m. The kit is built on a pre-coated 96-well microplate platform (12 x 8 well strips). An antibody specific for Epiregulin is immobilised on the plate; standards and samples are added, followed by a Biotinylated Anti-Epiregulin Antibody and HRP-Avidin conjugate. TMB substrate produces a blue colour that turns yellow upon addition of acidic stop solution, with absorbance read at 450 nm for quantification against a standard curve.

Kit components include a pre-coated 96-well microplate, lyophilised standard, Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers. Reported recovery values are: Serum (n=5) 80%-102% average 91%; EDTA Plasma (n=5) 81%-100% average 90%; Heparin Plasma (n=5) 80%-89% average 84%. Dilutional linearity data is provided for serum, EDTA plasma, and heparin plasma across dilutions of 1:2, 1:4, 1:8, and 1:16. Storage requirements are component-specific across +4°C and -20°C; the kit should not be used past its expiration date.