Rat ELAV Like Protein 1 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of rat HuR (ELAV Like Protein 1) in tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate configured as 12 x 8 well strips and employs a biotinylated detection antibody with HRP-avidin and TMB substrate, producing a colorimetric readout measured at 450 nm.

Reported performance includes a sensitivity of 0.066 ng/ml and an assay range of 0.156-10 ng/ml, with a total assay time of 4h 30m. Spike-and-recovery values across sample matrices are: serum (range 80-102%, average 91%), EDTA plasma (range 81-101%, average 91%), and heparin plasma (range 80-89%, average 84%). Dilution linearity data are provided for serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8 and 1:16. Kit components are stored at +4°C or -20°C as instructed, and the assay is intended for use with a colorimetric microplate reader.