Rat Dual Serine/Threonine And Tyrosine Protein Kinase ELISA Kit

This Sandwich ELISA kit from Antibodies.com enables the quantitative determination of Rat Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK/RIPK5) in tissue homogenates and other biological fluids. The assay uses a pre-coated 96-well microplate with an immobilised antibody specific for DSTYK/RIPK5 and employs the sandwich enzyme immunoassay technique, incorporating a biotinylated detection antibody and an HRP-Avidin conjugate. Signal is developed with TMB substrate and the enzymatic reaction is read at 450 nm in a colorimetric microplate reader.

The measuring range is 0.312-20 ng/ml with a sensitivity of <0.113 ng/ml, and the complete assay time is 4 hours 30 minutes. Sample validation data show average recovery of 91% for serum, 90% for EDTA plasma, and 84% for heparin plasma, with dilution linearity demonstrated from 1:2 to 1:16 across the same sample types.

Kit components include a pre-coated microplate, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, with storage conditions of +4°C or -20°C as appropriate for each component.