Rat Diamine Oxidase ELISA Kit

From Antibodies.com, this is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat D Amino Acid Oxidase in serum, plasma, tissue homogenates, cell lysates or other biological fluids. The assay uses a pre-coated microplate (12 x 8 well strips) with an immobilised antibody specific for the target, followed by a biotinylated detection antibody and an HRP-avidin conjugate, producing a colour change read as absorbance at 450nm.

According to the supplied product literature, the kit has a stated sensitivity of 1.33 ng/ml and a standard curve range of 3.12-200 ng/ml, with a total assay time of 4h 30m. A 1:2 dilution series is prepared from a 200 ng/ml stock down to 3.13 ng/ml. Reported recovery ranges across n=5 spiked samples are 80-102% (average 91%) for serum, 81-100% (average 90%) for EDTA plasma, and 80-89% (average 84%) for heparin plasma. The procedure involves sequential incubations at 37°C with detection reagents, TMB substrate development in the dark for 15-25 minutes, and an acidic stop solution, with the absorbance read on a colourimetric microplate reader.

Kit components include the pre-coated 96-well microplate (stored at -20°C), lyophilised standard (2 vials, -20°C), Detection Solution A and Detection Solution B (120µl each, -20°C), 30X Wash Buffer (20ml, +4°C), Sample Dilution Buffer (45ml, -20°C), TMB Substrate (9ml, +4°C), Stop Solution (6ml, +4°C), and adhesive plate sealers. Kit components should be stored at +4°C or -20°C as instructed and not used past the expiration date. The product is intended for research use only.