Rat Deiodinase, Iodothyronine, Type II ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of rat DIO2 (Deiodinase, Iodothyronine, Type II) in tissue homogenates or other biological fluids. The kit employs a pre-coated 96-well microplate arranged as 12 x 8 well strips and uses a colorimetric detection method read on a standard microplate reader at 450 nm.

The assay has a sensitivity of <0.24 ng/ml and a working range of 0.781–50 ng/ml. Total assay time is 4h 30m. Standards and samples are captured by an immobilized anti-DIO2 antibody, detected with a Biotinylated Anti-DIO2 Antibody followed by HRP-Avidin conjugate, and visualised using TMB substrate, with absorbance proportional to DIO2 concentration. Recovery in serum averaged 91% (range 80%–102%), in EDTA plasma 90% (range 81%–100%), and in heparin plasma 84% (range 80%–89%). Dilution linearity across 1:2 to 1:16 dilutions is also reported for these sample types.

Kit components include a pre-coated microplate, lyophilised standard, Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers. Components should be stored at +4°C or −20°C as instructed and the kit should not be used past its expiration date.