Rat Cytochrome P450 2E1 ELISA Kit

Manufactured by Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) kit is designed for the in vitro quantitative determination of rat Cytochrome P450 2E1 in tissue homogenates, cell lysates, and other biological fluids, and falls within the Assays and Kits product category.

The assay is run on a pre-coated 96-well microplate arranged as 12 x 8 well strips, with detection performed colorimetrically by reading absorbance at 450 nm. Quantitative performance is characterised by an assay range of 3.12-200 ng/ml and a sensitivity of <1.25 ng/ml, with a total assay time of 4 hours 30 minutes. Target analyte is captured by an antibody specific for Cytochrome P450 2E1 immobilised on the plate, then detected with a biotinylated anti-Cytochrome P450 2E1 antibody and an HRP-Avidin conjugate; TMB substrate yields a blue product that turns yellow on addition of acidic stop solution, with intensity proportional to analyte concentration.

Documented as suitable for cell lysates and tissue homogenates. Kit components include the pre-coated plate, lyophilised standard, detection solutions, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers. Components should be stored at +4°C or -20°C as instructed and not used past the stated expiration date.