Rat Cross Linked C-Telopeptide Of Type I Collagen ELISA Kit

This sandwich ELISA from Antibodies.com is designed for the in vitro quantitative determination of rat Cross Linked C-Telopeptide Of Type I Collagen in serum, plasma, or other biological fluids. The kit employs the sandwich enzyme immunoassay technique on a pre-coated 96-well microplate, using HRP-Avidin detection with a Biotinylated Anti-target Antibody, TMB substrate and measurement of absorbance at 450 nm in a colorimetric microplate reader.

The reported assay range is 0.156–10 ng/ml, with a sensitivity of <0.042 ng/ml. Total assay time is 4 hours 30 minutes. The density of yellow colour is proportional to the amount of analyte captured in each well, and the concentration is calculated by referring to a standard curve. Kit components should be stored at +4°C or -20°C as instructed and not used past the expiration date.

Sample validation data is provided. Average recovery values are 91% for serum (range 80–102%, n=5), 90% for EDTA plasma (range 81–100%, n=5), and 84% for heparin plasma (range 80–89%, n=5). Dilution linearity is also reported across these sample types at dilutions of 1:2, 1:4, 1:8, and 1:16.