Rat Coupling Factor 6 ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit enables the in vitro quantitative determination of rat Coupling Factor 6 (also known as ATP5J) in serum, plasma, tissue homogenates and other biological fluids. The assay uses a pre-coated 96-well microplate and a biotinylated anti-ATP5J antibody / HRP-avidin detection system with TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader. Sensitivity is 0.29 ng/ml and the standard curve spans 0.781–50 ng/ml, with a total assay time of 4h 30m.

Recovery in serum, EDTA plasma and heparin plasma ranges from 80% to 102%, with linearity values of 74–108% across dilution factors from 1:2 to 1:16. Kit components include a pre-coated microplate, lyophilized standard, Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and adhesive plate sealers, to be stored at +4°C or -20°C as instructed. The kit is intended for research use only.