Rat Copeptin ELISA Kit

Antibodies.com offers this sandwich ELISA for the in vitro quantitative determination of rat Vasopressin in serum, plasma or other biological fluids. A pre-coated 96-well microplate formatted as 12 x 8 well strips captures the analyte via an immobilized antibody; a biotinylated detection antibody, HRP-avidin conjugate and TMB substrate are then added sequentially, with the resulting colour read at 450 nm on a microplate reader.

Sensitivity is <5.9 pg/ml and the quantitative range is 15.6–1000 pg/ml. The protocol takes 4 hours 30 minutes, with components stored at +4°C or -20°C as instructed. Reported recovery averages 91% in serum (range 80–102%, n=5), 90% in EDTA plasma (81–100%, n=5), and 84% in heparin plasma (80–89%, n=5), alongside dilution-linearity data across 1:2 to 1:16 dilutions.