Rat Cocaine And Amphetamine Regulated Transcript ELISA Kit

From Antibodies.com, this Rat CART ELISA Kit is a sandwich enzyme-linked immunosorbent assay (sELISA) for the in vitro quantitative determination of rat Cocaine And Amphetamine Regulated Transcript in serum, plasma, tissue homogenates or other biological fluids. The pre-coated 96-well microplate (12 x 8 well strips) uses an immobilized CART-specific antibody; standards and samples are added, followed by a biotinylated anti-CART antibody, HRP-Avidin conjugate and TMB substrate, producing a blue product that turns yellow on addition of stop solution. Absorbance is read at 450 nm in a colorimetric microplate reader and CART concentration is calculated against the standard curve.

The assay has a sensitivity of <5.8 pg/ml and a working range of 15.6-1000 pg/ml, with a total run time of 4h 30m. Recovery was validated in serum (n=5, average 91%, range 80-102%), EDTA plasma (n=5, average 90%, range 81-100%) and heparin plasma (n=5, average 84%, range 80-89%), with consistent performance reported at sample dilutions of 1:2, 1:4, 1:8 and 1:16. Kit components should be stored at +4°C or -20°C as instructed and not used past the stated expiration date.