Rat Coagulation Factor VII ELISA Kit

Manufactured by Antibodies.com, this Sandwich ELISA kit is intended for the in vitro quantitative determination of rat Factor VII in plasma or other biological fluids. The assay uses a pre-coated microplate and employs the sandwich enzyme immunoassay technique, with a biotinylated anti-Factor VII detection antibody and an HRP-avidin conjugate. The enzymatic reaction is visualised using TMB substrate, and the absorbance is read at 450 nm with a colorimetric microplate reader.

Reported by the manufacturer, the quantitative assay has a standard range of 62.5-4000 pg/ml and a sensitivity of 22.3 pg/ml, with a total assay time of 4h 30m. Average recoveries from the manufacturer are 91% in serum (n=5, range 80-102%), 90% in EDTA plasma (n=5, range 81-99%) and 84% in heparin plasma (n=5, range 80-89%). Linearity is documented at dilutions of 1:2, 1:4, 1:8 and 1:16 across these sample types. Kit components should be stored at +4°C or -20°C as instructed, and the product should not be used past its expiration date.