Rat Chymase 1, Mast Cell ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of rat Mast Cell Chymase in serum, plasma, tissue homogenates, cell lysates, and other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) with an antibody specific for Mast Cell Chymase, followed by a biotinylated detection antibody, HRP-Avidin conjugate, and TMB substrate; absorbance is read at 450 nm on a colorimetric microplate reader. The product range is 1.56–100 ng/ml with a sensitivity of 0.57 ng/ml, and the total assay time is 4 hours 30 minutes.

Kit components include the pre-coated microplate, lyophilized standard (2 vials), Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers, with storage at +4°C or -20°C as indicated. Recovery in serum is reported as 80–102% (average 91%), in EDTA plasma as 81–100% (average 90%), and in heparin plasma as 80–89% (average 84%), based on n=5 per matrix.

Sample dilution linearity across 1:2 to 1:16 dilutions shows serum recoveries of 87–91% to 92–97%, EDTA plasma recoveries of 90–105% to 79–108%, and heparin plasma recoveries of 84–95% to 89–91% (n=5). The kit is suitable for serum, plasma, cell lysates, and tissue homogenates.