Rat Chemokine C-X-C-Motif Ligand 16 ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of rat CXCL16 (Chemokine C-X-C-Motif Ligand 16) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The assay uses the sandwich enzyme immunoassay technique with an antibody specific for CXCL16 pre-coated onto a 96-well microplate. Standards and samples are added to the wells, where CXCL16 is captured by the immobilized antibody and detected using a Biotinylated Anti-CXCL16 Antibody followed by an HRP-Avidin conjugate. TMB substrate is then used to visualise the reaction; the colour change is read at 450 nm in a microplate reader and the concentration of CXCL16 is calculated by reference to a standard curve.

The kit has a stated product range of 0.156-10 ng/ml and a sensitivity of 0.058 ng/ml. Reported average recovery values (n=5) are 91% for serum (range 83%-100%), 94% for EDTA plasma (range 90%-98%), and 95% for heparin plasma (range 89%-102%). Dilutional linearity data are provided at 1:2, 1:4, 1:8, and 1:16 dilutions for serum, EDTA plasma, and heparin plasma samples. The total assay time is 4 hours 30 minutes on a pre-coated microplate platform of 12 x 8 well strips, with colourmetric detection.

Kit components include a pre-coated microplate, lyophilised standard, Detection Solution A, Detection Solution B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers. Components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date. The kit is reactive with rat samples and is suitable for serum, plasma, cell lysates, tissue homogenates, and cell culture supernates.