Rat Cathepsin G ELISA Kit

From Antibodies.com, this sandwich ELISA is designed for the in vitro quantitative determination of rat Cathepsin G in tissue homogenates or other biological fluids. Listed under the Assays and Kits product category and rated for Rat reactivity, the assay uses a pre-coated microplate, a biotinylated anti-Cathepsin G detection antibody, an HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

The stated assay sensitivity is <0.057 ng/ml with a working range of 0.156–10 ng/ml, and the full protocol takes 4 h 30 min. Spike-and-recovery has been reported in serum (n=5, range 80–102%, average 91%), EDTA plasma (n=5, range 81–100%, average 90%) and heparin plasma (n=5, range 80–89%, average 84%). Dilution linearity has been assessed at 1:2, 1:4, 1:8 and 1:16 dilutions: serum 87–91%, 87–107%, 74–101% and 92–97%; EDTA plasma 90–105%, 84–101%, 90–101% and 79–108%; heparin plasma 84–95%, 92–105%, 82–105% and 89–91%. Components are intended for storage at +4°C or –20°C as instructed, and the kit should not be used past its expiration date.