Rat Carboxypeptidase A1, Pancreatic ELISA Kit

Antibodies.com provides a sandwich ELISA developed for the in vitro quantitative determination of rat Carboxypeptidase A in serum, plasma, or other biological fluids. The assay targets Carboxypeptidase A1, Pancreatic and is reactive with rat samples, falling within the Assays and Kits category.

Quantitative results are obtained by reading optical density at 450 nm on a colorimetric microplate reader and comparing against a standard curve. The detection range spans 1.56-100 U/L with a sensitivity of <0.52 U/L, and the full assay protocol completes in 4h 30m using a pre-coated 96-well microplate platform of 12 x 8 well strips.

Recoveries across 5 samples were 80%-102% (average 91%) in serum, 81%-100% (average 90%) in EDTA plasma, and 80%-89% (average 84%) in heparin plasma. The kit includes a pre-coated microplate, lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, stored at +4°C or -20°C according to the instructions.