Rat Bone Morphogenetic Protein 8A ELISA Kit

Antibodies.com supplies a sandwich ELISA for the in vitro quantitative determination of rat BMP8a/OP-2 in serum, plasma and other biological fluids, with reactivity confirmed for rat samples. The assay employs a pre-coated microplate to which standards and test samples are added; captured BMP8a/OP-2 is detected using a biotinylated anti-BMP8a/OP-2 antibody followed by an HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm on a colourmetric microplate reader.

According to the manufacturer, the assay range is 0.312–20 ng/ml with a sensitivity of <0.116 ng/ml, and the total assay time is 4 h 30 m. Reported average recoveries are 93% in serum (range 84–102%, n=5), 95% in EDTA plasma (range 91–100%, n=5) and 95% in heparin plasma (range 92–98%, n=5). Dilution linearity has been demonstrated at 1:2, 1:4, 1:8 and 1:16 across the same sample types, with the kit components to be stored at +4°C or –20°C as instructed.