Rat Bone Morphogenetic Protein 4 ELISA Kit

This Antibodies.com sandwich ELISA kit enables the in vitro quantitative determination of rat BMP4 (Bone Morphogenetic Protein 4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The assay uses a microtiter plate pre-coated with an antibody specific for BMP4. Standards and samples are added to the wells and bound BMP4 is detected using a biotinylated anti-BMP4 antibody followed by an HRP-avidin conjugate. The enzymatic reaction is visualised with TMB substrate, producing a blue product that turns yellow after addition of stop solution; absorbance is read at 450 nm. Total assay time is 4h 30m on a colorimetric microplate reader platform.

According to the manufacturer's specifications the quantitative range is 15.6-1000 pg/ml with a sensitivity of 6.1 pg/ml. Reported recovery values are 80%-102% for serum (average 91%), 81%-100% for EDTA plasma (average 90%) and 80%-89% for heparin plasma (average 84%). Dilution linearity data are provided for serum (87-91% at 1:2, 87-107% at 1:4, 74-101% at 1:8, 92-97% at 1:16), EDTA plasma (90-105% at 1:2, 84-101% at 1:4, 90-101% at 1:8, 79-108% at 1:16) and heparin plasma (84-95% at 1:2, 92-105% at 1:4, 82-105% at 1:8, 89-91% at 1:16). Kit components should be stored at +4°C or -20°C as instructed and the kit should not be used past its expiration date.

The kit is specific to rat reactivity, and BMP4 is also known by the synonyms BMP-2B, BMP-4, BMP2B, Bone morphogenetic protein 2B, Bone morphogenetic protein 4 and DVR4.