Rat Bone Morphogenetic Protein 1 ELISA Kit

A sandwich ELISA kit from Antibodies.com, classified under their Assays and Kits range, is designed for the in vitro quantitative determination of Bone Morphogenetic Protein 1 (BMP1/PCP) in rat serum, plasma, tissue homogenates and other biological fluids. The assay uses a pre-coated microplate format, a biotinylated detection antibody, HRP-conjugated avidin and TMB substrate, with absorbance read at 450 nm. The protocol is completed in 4 h 30 min.

Manufacturer specifications list a standard curve range of 31.2–2000 pg/ml and a sensitivity of <11.7 pg/ml. Sample-matrix performance figures are reported in serum (n=5; range 80–102%; average 91%), EDTA plasma (n=5; range 81–100%; average 90%) and heparin plasma (n=5; range 80–89%; average 84%). Dilution linearity values across 1:2, 1:4, 1:8 and 1:16 are also provided for the same three sample matrices.

A third-party listing for the same Antibodies.com product records an identical sensitivity of 11.7 pg/ml and a detection range of 31.25–2000 pg/ml, confirming sandwich ELISA methodology and rat reactivity in serum, plasma and tissue homogenates. The kit is supplied for research use only.