Rat Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of rat BNIP3 (Bcl2/Adenovirus E1B 19kDa Interacting Protein 3) in tissue homogenates, cell lysates and other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with detection via a biotinylated anti-BNIP3 antibody, HRP-avidin conjugate and TMB substrate solution, with absorbance read at 450 nm in a colorimetric microplate reader.

Reported performance includes a sensitivity of 0.228 ng/ml and an assay range of 0.625–40 ng/ml, with a total assay time of 4 hours 30 minutes. Spike-and-recovery values stated in the product insert average 91% for serum (range 80–102%, n=5), 90% for EDTA plasma (range 81–100%, n=5) and 84% for heparin plasma (range 80–89%, n=5). Kit components include a lyophilized standard, Detection Solutions A and B, a 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and adhesive plate sealers, with components stored at +4°C or -20°C as instructed.