Rat ATPase, Na+/K+ Transporting Alpha 1 Polypeptide ELISA Kit

Antibodies.com supplies a sandwich enzyme-linked immunosorbent assay (sELISA) for the in vitro quantitative determination of rat Sodium Potassium ATPase in tissue homogenates or other biological fluids.

An antibody specific for the target is pre-coated onto a microplate. Standards and samples are added, followed by a biotinylated detection antibody and an HRP-avidin conjugate; TMB substrate produces a colour change read at 450 nm on a colorimetric microplate reader. The reported standard curve range is 0.156–10 ng/ml with a sensitivity of <0.062 ng/ml, and the assay takes 4h 30m. Components are stored at +4°C or –20°C. Reactivity is rat.

Product literature documents recovery and dilution linearity for serum, EDTA plasma and heparin plasma, reporting averages of 91% (serum), 91% (EDTA plasma) and 84% (heparin plasma) across the tested samples.