Rat Aquaporin 5 ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit enables the in vitro quantitative determination of rat Aquaporin 5 in tissue homogenates and other biological fluids. The assay uses a pre-coated microplate and an HRP-based colorimetric readout, with absorbance measured at 450 nm against a standard curve. The product range is 0.312-20 ng/ml and the kit achieves a sensitivity of <0.062 ng/ml. Total assay time is 4 hours 30 minutes from sample addition to final readout. Kit components are stored at +4°C or -20°C as instructed in the protocol; the kit should not be used past its expiration date.

The workflow uses a biotinylated anti-Aquaporin 5 detection antibody together with an HRP-Avidin conjugate, followed by TMB substrate development. The enzymatic reaction produces a blue-coloured product that converts to yellow upon addition of acidic stop solution, with the intensity of the yellow colour proportional to the amount of Aquaporin 5 captured in each well. The manufacturer also provides recovery and linearity data for serum, EDTA plasma and heparin plasma.