Rat Angiotensin II Receptor 1 ELISA Kit

From Antibodies.com, this sandwich ELISA is designed for the in vitro quantitative determination of rat Angiotensin II Type 1 Receptor in tissue homogenates or other biological fluids. The assay uses a pre-coated microplate and a biotinylated detection antibody, followed by HRP-Avidin and TMB substrate; absorbance is read at 450 nm on a colorimetric microplate reader.

Manufacturer-quoted performance figures include an assay range of 0.156-10 ng/ml, a sensitivity of 0.053 ng/ml, and a total assay time of 4h 30m. Reported mean recoveries are 91% for serum (range 80%-102%, n=5), 90% for EDTA plasma (range 81%-100%, n=5), and 84% for heparin plasma (range 80%-89%, n=5). Dilution linearity is reported across 1:2 to 1:16 dilutions for the same three sample types.

Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.