Rat Angiopoietin 2 ELISA Kit

From Antibodies.com, this sandwich-format ELISA kit enables in vitro quantitative determination of rat Angiopoietin 2/ANG2 in serum, plasma, or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with colorimetric readout at 450 nm on a standard microplate reader. Captured analyte is detected using a Biotinylated Anti-Angiopoietin 2/ANG2 antibody, HRP-Avidin conjugate, and TMB substrate, producing a yellow product whose intensity is proportional to ANG2 concentration.

Stated performance specifications include a measuring range of 0.187–12 ng/ml and a sensitivity of <0.09 ng/ml, with a total assay time of 4h 30m. Reported spike-and-recovery values are 91% on average in serum (range 80–102%), 91% in EDTA plasma (range 81–101%), and 84% in heparin plasma (range 80–89%), with corresponding dilution-linearity data given across 1:2 to 1:16 dilutions. The kit includes a pre-coated plate, lyophilized standard, Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers, with storage at +4°C or -20°C as indicated and a warning not to use past the kit's expiration date.