Rat Amiloride Sensitive Sodium Channel Subunit Alpha ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of rat Epithelial Sodium Channel alpha (Amiloride Sensitive Sodium Channel Subunit Alpha) in serum, plasma, tissue homogenates, or other biological fluids. The assay is run on a pre-coated microplate with colorimetric detection, where absorbance is read at 450 nm and target concentration is calculated against a standard curve.

Reported performance includes a detection range of 0.156–10 ng/ml and a sensitivity of <0.059 ng/ml, with a total assay time of approximately 4 hours 30 minutes. The workflow uses a biotinylated anti-target detection antibody followed by an HRP-avidin conjugate and TMB substrate; the resulting yellow product is proportional to the amount of target captured in each well.

Validation data shows recovery averaging 91% in serum (range 83%–100%, n=5), 94% in EDTA plasma (90%–98%, n=5), and 95% in heparin plasma (89%–102%, n=5), with linearity confirmed across 1:2, 1:4, 1:8, and 1:16 dilutions for all three sample matrices. Kit components should be stored at +4°C or −20°C as indicated, and the product should not be used past its expiration date.