Rat Alpha-Fetoprotein ELISA Kit

Quantitative measurement of Alpha-Fetoprotein (AFP) in rat specimens is supported by this immunoassay kit from Antibodies.com. AFP is a 70 kDa oncofetal glycoprotein expressed during fetal development; in adult rats, elevated circulating AFP is a recognized biomarker for hepatocellular injury, hepatic regeneration, and liver tumor models.

The kit employs a paired-antibody format in which AFP is captured between an immobilized plate-bound antibody and a labeled detection antibody, then quantified against a standard curve generated from calibrated AFP reference standards. Reagents are supplied for 96 tests in a microplate layout, with components that include a precoated plate, detection antibody concentrate, AFP reference standards, wash buffer, and substrate.

The protocol is suited for rat serum, rat plasma, and tissue culture supernatant from rat hepatocyte or hepatoma cell models, and is read spectrophotometrically after addition of stop solution. Reagents should be refrigerated upon arrival and brought to room temperature before each assay.

Laboratories in toxicology, hepatology, and cancer biology use this AFP ELISA to monitor hepatic damage after chemical exposure, track tumor burden in experimental hepatoma models, and characterize developmental AFP expression in neonatal rats. Researchers should consult the included product insert for validated sample types, expected working range, and recommended dilution factors for their specific application. Antibodies.com supplies the kit with technical documentation covering assay principle, reagent preparation, and standard curve generation.