Rat Albumin ELISA Kit

Manufactured by Antibodies.com, this competitive Enzyme-Linked Immunosorbent Assay (cELISA) is designed for the in vitro quantitative determination of rat Albumin in serum, plasma or other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) and a biotinylated anti-albumin antibody, with detection via an HRP-avidin conjugate and TMB substrate, read at 450 nm on a colorimetric microplate reader. The quoted detection range is 3.12–200 µg/ml and sensitivity is 0.93 µg/ml. Total assay time is 2h 30m.

Manufacturer validation data report the following average recovery rates in serum, EDTA plasma, and heparin plasma, respectively: 91% (range 80%–102%), 90% (range 81%–100%), and 84% (range 80%–89%). Linearity data for serum, EDTA plasma, and heparin plasma samples (n=5) at dilutions of 1:2, 1:4, 1:8, and 1:16 are also provided. Kit components include a lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, to be stored at +4°C or –20°C as instructed. The product is sold for laboratory research use only.