Rat Adrenergic Receptor Beta 2 ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of rat beta-2 Adrenergic Receptor in tissue homogenates and other biological fluids, including serum, EDTA plasma and heparin plasma. The assay employs a pre-coated 96-well microplate with immobilised capture antibody specific for the target. Standards and samples are added, followed by a biotinylated anti-receptor detection antibody and an HRP-avidin conjugate. TMB substrate produces a colour change read at 450 nm in a microplate reader, with target concentration calculated against the standard curve.

Sensitivity is reported as <0.058 ng/ml with an assay range of 0.156–10 ng/ml. Mean recovery values were 91% for serum (range 83–99%, n=5), 89% for EDTA plasma (range 80–99%, n=5) and 90% for heparin plasma (range 82–99%, n=5). The total assay time is approximately 4 hours 30 minutes. Kit components should be stored at +4 °C or –20 °C as instructed, and the kit should not be used past the stated expiration date.