Rat Adiponectin Receptor 1 ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of rat adiponectin receptor 1 (ADIPOR1) in serum, plasma or other biological fluids. The assay employs a 96-well microtiter plate pre-coated with an antibody specific for ADIPOR1. Standards and test samples are added, and ADIPOR1 present in each sample binds to the immobilised antibody. After incubation and washing, biotinylated anti-ADIPOR1 antibody is added, followed by HRP-avidin conjugate. TMB substrate solution then produces a blue colour that turns yellow upon addition of acidic stop solution; the density of yellow, read at 450 nm, is proportional to the amount of ADIPOR1 captured.

Key specifications include a sensitivity of <0.71 ng/ml, an assay range of 1.56–100 ng/ml, and a total assay time of 4h 30m. The plate is read on a colorimetric microplate reader. Reported recovery values (n=5) are: serum 80%–102% (average 91%); EDTA plasma 81%–101% (average 91%); heparin plasma 80%–89% (average 84%). Dilution linearity at 1:2, 1:4, 1:8 and 1:16 was: serum 87–91%, 87–107%, 74–101%, 92–97%; EDTA plasma 90–105%, 84–101%, 90–101%, 79–108%; heparin plasma 84–95%, 92–105%, 82–105%, 89–91%.

Kit components include a pre-coated 96-well microplate (12 x 8 well strips), lyophilised standard (2 vials), detection solutions A and B (120 µl each), 30X wash buffer (20 ml), sample dilution buffer (45 ml), TMB substrate (9 ml), stop solution (6 ml) and plate sealers. Components should be stored at +4°C or –20°C as instructed and not used past the expiration date.