Rat A Disintegrin And Metalloprotease 8 ELISA Kit

A sandwich ELISA kit from Antibodies.com for the quantitative determination of rat A Disintegrin And Metalloprotease 8 in serum, plasma, tissue homogenates and other biological fluids. The 96-well pre-coated microplate (12 x 8 well strips) employs a biotinylated detection antibody and HRP-avidin conjugate, with TMB substrate catalysed to a blue product that turns yellow after stop solution addition. Absorbance is read at 450 nm and concentration is calculated from a standard curve. Total assay time is 4 hours 30 minutes. Manufacturer-stated sensitivity is 6.35 pg/ml and the assay range is 15.6-1000 pg/ml. Reported average recoveries range from 84% to 91% across serum, EDTA plasma and heparin plasma samples (n=5). Components include lyophilised standard, detection solutions A and B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution and plate sealers, with storage at +4°C or -20°C as instructed.