Rabbit S100 Calcium Binding Protein A11 ELISA Kit

Manufactured by Antibodies.com, this Sandwich ELISA kit enables the in vitro quantitative determination of rabbit S100 Calcium Binding Protein A11 (S100A11) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The assay uses the sandwich enzyme immunoassay technique on a pre-coated 96-well microplate arranged as 12 x 8 well strips. Standards and samples are added to the wells, captured S100A11 is bound by a Biotinylated Anti-S100A11 Antibody, and an HRP-Avidin conjugate together with TMB substrate is used to generate signal that produces a yellow product after addition of stop solution. Absorbance is read at 450 nm on a colorimetric microplate reader, with colour intensity proportional to the amount of S100A11 captured.

Reported performance includes a quantitative measuring range of 0.625-40 ng/ml and a sensitivity of 0.15 ng/ml, with a total assay time of 4h 30m. Spike-and-recovery has been evaluated in serum (average 91%, n=5), EDTA plasma (94%, n=5) and heparin plasma (95%, n=5), and dilution linearity has been assessed across 1:2, 1:4, 1:8 and 1:16 dilutions of the same sample matrices. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past the expiration date.