Rabbit Platelet Derived Growth Factor Subunit A ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of rabbit PDGF AA (Platelet Derived Growth Factor Subunit A) in serum, plasma or other biological fluids. The assay employs a pre-coated microplate configured as 12 x 8 well strips, with detection achieved colorimetrically using a TMB substrate read at 450 nm. Standards and samples are captured by an immobilised antibody specific for PDGF AA, followed by a Biotinylated Anti-PDGF AA Antibody and an HRP-Avidin conjugate.

The reported assay range is 31.2-2000 pg/ml, with a sensitivity of <10.8 pg/ml, and a total assay time of 4h 30m. The kit has been validated for serum and plasma samples, with reported recovery ranges of 80-102% for serum, 81-100% for EDTA plasma, and 80-89% for heparin plasma (n=5 per sample type). Kit components include a pre-coated 96-well microplate, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, with storage instructions calling for -20°C for most reagents and +4°C for wash buffer, TMB substrate, and stop solution.