Rabbit Osteocalcin ELISA Kit

From Antibodies.com, this sandwich ELISA is designed for the in vitro quantitative determination of rabbit Osteocalcin in serum, plasma, tissue homogenates, or other biological fluids. The quantitative assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with colorimetric detection read at 450 nm on a standard microplate reader.

Performance specifications include an assay range of 0.312-20 ng/ml, a sensitivity of <0.113 ng/ml, and a total assay time of 4 hours 30 minutes. Spike-and-recovery testing showed serum (n=5) at 80-102% (average 91%), EDTA plasma (n=5) at 81-99% (average 90%), and heparin plasma (n=5) at 80-89% (average 84%). Dilution linearity was validated for serum, EDTA plasma, and heparin plasma across 1:2 to 1:16 dilutions. Kit components should be stored at +4°C or -20°C as indicated and the kit should not be used past its expiration date.