Rabbit Interleukin 1 Receptor Antagonist ELISA Kit

From Antibodies.com, this sandwich ELISA kit enables the in vitro quantitative determination of rabbit Interleukin 1 Receptor Antagonist (IL-1RA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids [C0][S1]. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) and relies on a biotinylated anti-IL-1RA detection antibody with an HRP-avidin conjugate; the enzymatic reaction is visualised using TMB substrate and read at 450 nm on a colorimetric microplate reader [S1].

The stated product range is 31.2–2000 pg/ml with a sensitivity of 12.8 pg/ml, and the total assay time is approximately 4 hours 30 minutes [S1]. Kit components should be stored at +4°C or -20°C as instructed and not used past the expiration date [S1]. In validation testing, mean recoveries were 91% for serum (range 80–102%, n=5), 90% for EDTA plasma (range 81–100%, n=5) and 84% for heparin plasma (range 80–89%, n=5), with dilutional linearity reported across 1:2 to 1:16 for each sample type [S1].