Rabbit Interleukin 1 Alpha ELISA Kit

A sandwich ELISA from Antibodies.com designed for the in vitro quantitative determination of rabbit IL-1 alpha in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The kit uses a pre-coated 96-well microplate (12 x 8 well strips) and the sandwich enzyme immunoassay principle: an antibody specific for IL-1 alpha is immobilised on the plate, samples and standards are added, followed by a Biotinylated Anti-IL-1 alpha Antibody and an HRP-Avidin conjugate. Bound IL-1 alpha is visualised using TMB substrate, with the colour change stopped by acidic stop solution and quantified by absorbance at 450 nm.

According to the manufacturer, the quantitative measuring range is 15.6-1000 pg/ml, the sensitivity is <5.4 pg/ml, and the total assay time is 4h 30m. Reported average recovery (n=5) is 91% for serum (range 83%-100%), 96% for EDTA plasma (range 90%-103%) and 95% for heparin plasma (range 89%-102%). Linearity has been demonstrated across serial dilutions (1:2, 1:4, 1:8 and 1:16) for serum (83-110%, 79-100%, 90-98%, 82-108%), EDTA plasma (90-98%, 90-105%, 73-109%, 87-91%) and heparin plasma (88-103%, 77-105%, 95-98%, 87-110%).

Kit contents include Pre-Coated 96 Well Microplate strips, Lyophilized Standard, Detection Solutions A and B, Wash Buffer (30X), Sample Dilution Buffer, TMB Substrate, Stop Solution and adhesive plate sealers, with components stored at +4°C or -20°C as indicated. The assay is intended for use with a colorimetric microplate reader and should not be used past the kit's expiration date.