Rabbit Intercellular Adhesion Molecule 1 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com is intended for the in vitro quantitative determination of rabbit Intercellular Adhesion Molecule 1 (ICAM1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The platform is a pre-coated 96-well microtiter plate configured as 12 x 8 well strips, with detection via a biotinylated anti-ICAM1 antibody, HRP-avidin conjugate and TMB substrate, read at 450 nm on a colorimetric microplate reader.

The stated product range is 125-8000 pg/ml with a sensitivity of <57 pg/ml, and the total assay time is 4 hours 30 minutes. Spike-and-recovery performance averaged 91% in serum (range 80-102%, n=5), 90% in EDTA plasma (81-100%, n=5) and 84% in heparin plasma (80-89%, n=5). Dilution linearity is reported across 1:2, 1:4, 1:8 and 1:16 sample dilutions for each matrix.

Kit contents include the pre-coated microplate, lyophilised standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers. Storage conditions vary by component: the plate, standard, detection solutions and sample dilution buffer are stored at -20°C, while the wash buffer, TMB substrate and stop solution are stored at +4°C.