Rabbit Bone Morphogenetic Protein 4 ELISA Kit

From Antibodies.com, this Sandwich ELISA kit enables in vitro quantitative determination of rabbit BMP4 (Bone Morphogenetic Protein 4) in serum, plasma, tissue homogenates, and other biological fluids [S1]. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and follows the sandwich enzyme immunoassay technique, with absorbance measured at 450nm on a colorimetric microplate reader [S1]. Total assay time is 4 hours 30 minutes [S1].

Detection uses a biotinylated anti-BMP4 antibody with HRP-avidin conjugate, and TMB substrate produces a colour change proportional to BMP4 concentration, which is read against a standard curve [S1]. The working assay range is 15.6-1000 pg/ml with a sensitivity of 5.8 pg/ml [S1].

Kit components include a pre-coated 96-well microplate, lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers, stored at +4°C or -20°C as instructed [S1]. In validation testing (n=5), average recovery was 91% for serum (range 80-102%), 90% for EDTA plasma (range 81-100%), and 84% for heparin plasma (range 80-89%) [S1]. Dilution linearity at 1:2, 1:4, 1:8, and 1:16 dilutions showed recovery ranges of 87-107% for serum, 84-108% for EDTA plasma, and 82-105% for heparin plasma [S1].