Rabbit Apolipoprotein A1 ELISA Kit

From the Antibodies.com catalogue of assays and kits, this competitive ELISA kit is designed for the in vitro quantitative determination of rabbit Apolipoprotein A I in serum, plasma, or other biological fluids. The procedure uses the competitive enzyme immunoassay technique on a pre-coated 96-well microtiter plate, employing an HRP-Avidin conjugate and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

Quantitative performance is supported by a working range of 31.2–2000 ng/ml and a sensitivity of <9.35 ng/ml, with a total assay time of approximately 2 h 30 min. Validation data supplied with the kit reports spiked recovery averages of 91% in serum, 90% in EDTA plasma and 84% in heparin plasma (n=5), together with linearity data across 1:2 to 1:16 dilutions for each sample type.

Kit components include a pre-coated 96-well microplate (12 x 8 well strips), lyophilised standard, Detection Solution A, Detection Solution B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers, with storage at +4°C or -20°C as indicated for each item.