Rabbit Angiotensin II ELISA Kit

Competitive enzyme-linked immunosorbent assay (cELISA) from Antibodies.com, intended for the in vitro quantitative determination of rabbit Angiotensin II in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The kit uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, and the competitive immunoassay format means that Angiotensin II in samples or standards competes with immobilised antigen for binding to a biotinylated anti-Angiotensin II antibody, with detection via HRP-conjugated avidin and a TMB substrate read at 450 nm.

Antibodies.com specifies a product range of 31.2–2000 pg/ml and a sensitivity of <9.66 pg/ml, with a total assay time of 2 h 30 min and results captured on a colorimetric microplate reader. Reported recovery values are 80%–102% (average 91%, n=5) for serum, 81%–100% (average 90%, n=5) for EDTA plasma and 80%–89% (average 84%, n=5) for heparin plasma. Kit components are stored at +4 °C or −20 °C as indicated on the individual component labels.